Fluorimetric Estimation of Estrogens in Bovine Urine

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Automated hydrolysis of total estrogens in urine from pregnant women.

This method for the hydrolysis of estrogens completes the automated analysis of estrogens in pregnancy urine described by Muir, Ua Conaill, and Ryan [Steroids 13, 719 (1969)]. Pregnancy urines can be processed from the sampling of urine to the recording of fluorescence intensity in 30 mm, at a rate of 20 samples per hour. The presence of glucose does not de crease the apparent hydrolysis products.

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Hopkins, R. H. (1943). Nature, Lond., 152, 274. Irwin, J. 0. (1944). In the Press. Lyle, T. K., Macrae, T. F. & Gardiner, P. A. (1944). Lancet, 1, 393. Macrae, T. F., Henry, K. M. & Kon, S. K. (1943). Biochem. J. 37, 225. Sebrell, W. H. & Butler, R. E. (1938). Pubi. HUh Rep., Wash., 53, 2282. Snell, E. E. & Strong, F. M. (1939). Industr. Engng Chem. (Anal. ed.), 11, 346. Williams, R. D., Mason,...

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Homogeneous luminescence immunoassay for total estrogens in urine.

We describe an homogeneous luminescence immunoassay for "total" estrogens in enzymically hydrolyzed urine from nonpregnant women. The antiserum, raised against estriol-16,17- dihemisuccinate conjugated to bovine serum albumin, specifically bound the C-19 steroids carrying the estrogen-characteristic phenolic group. 17 beta-Estradiol conjugated with aminobutylethyl isoluminol was used to monitor...

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ژورنال

عنوان ژورنال: Journal of Dairy Science

سال: 1956

ISSN: 0022-0302

DOI: 10.3168/jds.s0022-0302(56)94723-3